Journal: Transboundary and Emerging Diseases
Article Title: Toxoplasma gondii KCR is a Noncanonical Modulator of CSF2 Signaling that Targets the CSF2Rα–JAK2/STAT5 Axis
doi: 10.1155/tbed/8426765
Figure Lengend Snippet: Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse mAb, His‐tag mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.
Article Snippet: Flag‐tag mouse monoclonal antibody (mAb) (#M20008), His‐tag mouse mAb (# M20001 ), and mouse IgG (#B30010M) were purchased from Abmart Biotech, Inc. (Shanghai, China).
Techniques: Immunoprecipitation, Transfection, FLAG-tag, Western Blot, Marker, Purification